Holiday revellers drove along a Fraser Island beach towards a landing Queensland rescue chopper and shone headlights and a spotlight directly into the cockpit as the crew tried to land.
The AGL Action Rescue Helicopter was preparing to land on Orchid Beach to pick up a patient who had lacerations to his left arm after putting it through a plate glass window.
Revellers in four-wheel-drives roared up the beach and shone the lights which obscured the vision of the crew members, said pilot Peter Potroz.
He flashed the helicopter's spotlight at them in the hope the beach-goers would stop, but instead they persisted.
"We knew the patient we were airlifting had lost a significant amount of blood and needed urgent medical treatment from our flight paramedic," Mr Potroz said in a statement.
"It made the landing difficult and more timely than usual, but we remained calm and concentrated on the task at hand.
"Fortunately we got to him in time."
The incident has been reported to police.
Since Christmas the AGL Action Rescue Helicopter has flown to Fraser Island 16 times to help more than a dozen people, including children suffering burns, stings and serious medical conditions.