After Friday, myki will be the only ticket in town.
Melbourne's public transport ticket Metcard will be relegated to the history books to make way for the new $1.35 billion myki smartcard system.
Metcard sales on trams and buses and Metcard use on trains, trams and buses ends on Friday, with myki the only ticket that can be used from the first service on Saturday.
This means commuters will need a myki card before boarding and there will be no top-up facilities on trams.
Cards can be topped up online, at a myki retail outlet or 7-Eleven, at a Premium Station ticket office or at a myki machine, found at all Metro train stations and some tram stops and bus interchanges.
Meanwhile, the Public Transport Users Association has welcomed the reintroduction of paper tickets as an alternative to myki on Mornington Peninsula buses.
The paper ticket will only be available to seniors card holders on routes 787 and 788.
Their introduction comes after it emerged the seniors daily fare covering that region would no longer be available under myki, forcing commuters to pay between $5.42 and $9.02 for a day's travel compared to the current $3.80.
The seniors ticket, which can be bought from the bus driver, is valid for one day's travel on the peninsula and in metropolitan Melbourne.
Public Transport Users Association president Tony Morton said the paper seniors ticket is "proof positive" that paper tickets can be made to work alongside myki and that two-hour and daily short-term tickets should be retained.
"Given the system can accommodate this option, it should be made available to occasional travellers, visitors and tourists who neither want nor need to pay $6 for a myki card that won't be used again for months," he said.
Regional centres such as Geelong will receive myki in the first half of next year.