The Victorian Government has announced that 370 properties in Melbourne's inner north will be affected by plans to construct the East West Link.
The government has released detailed mapping of the project's first stage.
It shows a band of properties to be compulsorily acquired near Hoddle Street and Alexandra Parade.
They include 118 surface properties and 92 homes.
Another 258 properties where the proposed tunnel will pass underneath will also be eligible for some compensation.
"Assistance with relocation expenses, assistance with relocating children to other schools, a whole range of other one- by-one case-by-case processes," Premier Denis Napthine said.
"We are confident we can deal with each and every affected household."
The government says final acquisition notices will not be sent out until next year.
It has also released new details for the tunnel section passing underneath Royal Park in Parkville.
Premier Napthine says the land acquisition process will be fair for affected residents.
"We are confident that we can deal with each and every affected household in a compassionate, caring and appropriate way, that gives a positive outcome," he said.
"Ultimately people can take the matter to VCAT and the courts."
Labor's Tim Pallas says the opposition is not against compulsory acquisition.
But he says residents who stand to lose their homes have been treated badly.
"They've come into these communities, they've caused great grief, and in so doing they've basically told them they have no certainty in terms of their future," he said.
"They don't know when their properties will be acquired, they don't know for what purpose."
Keith Fitzgerald lives on Bendigo Street in Collingwood and has received a letter from the government informing him his property was likely to be required for the construction of the tollway..
He says he was devastated by the the news.
"I don't want to move, I've been here all my life....It's one of the best areas in Melbourne," he said.
Helen Bonanno is another affected Collingwood resident and has vowed to fight to keep her home.
"This is my home and is my life. I'm a bit too old to look for another house," she said.
"I don't know how they're going to compensate, I've never heard a good thing about compensation."