Queensland Rail has reopened the train line north of Bundaberg, allowing freight and passengers trains to travel between Brisbane and Cairns.
Parts of the track washed away in the wake of ex-tropical cyclone Oswald.
QR is warning of delays while crews repair overhead power lines and track signals.
Two flood-damaged level crossings at North Bundaberg will be manually operated until repairs are finalised.
There are still disruptions to the Rockhampton Tilt Train service.